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Characterization of soil low-molecular-weight organic acids in the Karst rocky desertification region

Xiaoliang LI, Xiaomin CHEN, Xia LIU, Lianchuan ZHOU, Xinqiang YANG

《环境科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 195-203 doi: 10.1007/s11783-012-0391-1

摘要： Soil low-molecular-weight (LMW) organic acids play important roles in the soil-forming process and the cycling of nutrients in Karst regions. In this study, we quantified the contents of LMW organic acids (including lactate, acetate, formate, malate, and oxalate) in soil solution over the Karst region of Guizhou Province, China using ion chromatography. The concentration of total LMW organic acids in topsoil solution ranged from 0.358 to 1.823 μmol·g , with an average of 0.912 μmol·g . The mean concentrations of lactate, acetate, formate, malate, and oxalate were 0.212±0.089, 0.302±0.228, 0.301±0.214, 0.014±0.018 and 0.086±0.118 μmol·g , respectively. There were also significant difference in the contents of these acids among four phases of rocky desertification, and their concentrations decreased with the aggravation of rocky desertification. The concentrations of the LMW organic acids were significantly positive correlated each other. Significant positive correlations were also observed among individual LMW organic acids in soil solution, and between them and soil available P, available K, exchangeable Ca, respectively. Furthermore, the concentrations of LMW organic acids were significantly positively correlated with inorganic anions (chlorides, nitrates, and sulfates) in Karst topsoil solution. Therefore, the concentrations of soil LMW organic acids might be one of driving force in the Karst rock desertification process in Guizhou Province.

关键词： Karst rocky desertification     low-molecular-weight (LMW) organic acids     distribution characteristics     soil    

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Effects of a dynamic membrane formed with polyethylene glycol on the ultrafiltration of natural organic

Boksoon KWON, Noeon PARK, Jaeweon CHO,

《环境科学与工程前沿（英文）》 2010年 第4卷 第2期   页码 172-182 doi: 10.1007/s11783-010-0002-y

摘要： The formation of a dynamic membrane (DM) was investigated using polyethylene glycol (PEG) (molecular weight of 35000 g/mol, concentration of 1 g/L). Two natural organic matters (NOM), Dongbok Lake NOM (DLNOM) and Suwannee River NOM (SRNOM) were used in the ultrafiltration experiments along with PEG. To evaluate the effects of the DM with PEG on ultrafiltration, various transport experiments were conducted, and the analyses of the NOM in the membrane feed and permeate were performed using high performance size exclusion chromatography, and the pore size distribution ( PSD) and molecular weight cut off ( MWCO) were determined. The advantages of DM formed with PEG can be summarized as follows: (1) PEG interferes with NOM transmission through the ultrafiltration membrane pores by increasing the retention coefficient of NOM in UF membranes, and (2) low removal of NOM by the DM is affected by external factors, such as pressure increases during UF membrane filtration, which decreases the PSD and MWCO of UF membranes. However, a disadvantage of the DM with PEG was severe flux decline; thus, one must be mindful of both the positive and negative influences of the DM when optimizing the UF performance of the membrane.

关键词： dynamic membrane     natural organic matters     ultrafiltration membrane performance     effective PSD     effective molecular weight cutoff    

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Effects of organic acids on Cd adsorption and desorption by two anthropic soils

Jingui WANG, Jialong LV, Yaolong FU

《环境科学与工程前沿（英文）》 2013年 第7卷 第1期   页码 19-30 doi: 10.1007/s11783-012-0424-9

摘要： The objective of this experiment was to study the effects of malic, tartaric, oxalic, and citric acid on the adsorption and desorption characteristics of Cd by two typical anthropic soils (lou soil and irrigation-silted soil) in North-west China. Cadmium adsorption and desorption were studied under a range of temperatures (25°C, 30°C, 35°C, 40°C), organic acid concentrations (0.5–5.0 mmol·L ), and pH values (2–8). The results showed that the Cd adsorption capacity of the lou soil was significantly greater than that of the irrigation-silted soil. Generally, Cd adsorption increased as the temperature increased. In the presence of NaNO , the adsorption of Cd was endothermic with Δ values of 31.365 kJ·mol for lou soil and 28.278?kJ·mol for irrigation-silted soil. The endothermic reaction indicated that H bonds were the main driving force for Cd adsorption in both soils. However, different concentrations of organic acids showed various influences on the two soils. In the presence of citric acid, chemical adsorption and van der Waals interactions were the main driving forces for Cd adsorption rather than H bonds. Although the types of organic acids and soil properties were different, the effects of the organic acids on the adsorption and desorption of Cd were similar in the two soils. The adsorption percentage of Cd generally decreased as organic acid concentrations increased. In contrast, the adsorption percentage increased as the pH of the initial solution increased. The exception was that adsorption percentage of Cd increased slightly as oxalic acid concentrations increased. In contrast, the desorption percentage of Cd increased with increasing concentrations of organic acids but decreased as the initial solution pH increased.

关键词： adsorption     desorption     cadmium     organic acids     temperature     anthropic soil    

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Factors controlling

Chengkun WANG, Xiaojian ZHANG, Chao CHEN, Jun WANG

《环境科学与工程前沿（英文）》 2013年 第7卷 第2期   页码 151-157 doi: 10.1007/s11783-013-0482-7

摘要： The formation of cancinogenic nitrosamines, esp. -nitrosodimethylamine (NDMA) in water and wastewater treatment plants has drawn much attention in recent years. Dissolved organic matter from the transported Luan River water as water source of Tianjin was fractionated with different XAD resins and a series of ultra-filtration membranes with molecular weight (MW) cut-offs of 5k Da, 3k Da, and 1k Da, respectively. The NDMA yields from the raw water and each fraction were measured to investigate their role in NDMA yield. Results indicated that the hydrophilic fraction had a higher NDMA yield than those of hydrophobic fraction and transphilic fraction. The fraction with MW below 1k Da had a higher NDMA yield than that with larger MW. NDMA formation increased as the dissolved organic carbon (DOC) to dissolved organic nitrogen (DON) ratio decreased, which indicated that DON might serve as the real important precursor for NDMA. The correlation between NDMA yield and specific ultraviolet absorbance at 254 nm (SUVA ) suggested that the latter might not represent the specific precursors for NDMA in the water. Besides the water quality, the influences of pH, disinfectant dosage, and disinfection contact time on the formation of NDMA were also examined. These results will help water treatment plants establish measures to control this harmful disinfection by-product.

关键词： N-nitrosodimethylamine (NDMA)     disinfection by-product     dissolved organic nitrogen (DOC)     hydrophilic     molecular weight (MW)     specific ultraviolet absorbance at 254 nm (SUVA254)    

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Characterization of the dissolved organic matter in sewage effluent of sequence batch reactor: the impact

Jin GUO, Feng SHENG, Jianhua GUO, Xiong YANG, Mintao MA, Yongzhen PENG

《环境科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 280-287 doi: 10.1007/s11783-011-0336-0

摘要： Dissolved organic matter (DOM) transformation in sequence batch reactor (SBR) fed with carbon sources of different biodegradability was investigated. During the biologic degradation process, the low molecular weight (MW) fraction (<1 kDa) gradually decreased, while the refractory compounds with higher aromaticity were aggregated. Size exclusion chromatography (SEC) and fluorescence of excitation emission matrices (EEM) demonstrated that more biopolymers (polysaccharides or proteins) and humic-like substances were presented in the extracellular polymeric substance (EPS) extracted from the SBR fed with sodium acetate or glucose, while the EPS from SBR fed with slowly biodegradable dissolved organic carbon (DOC) substrate-starch had relatively less biopolymers. Comparing the EfOM in sewage effluent of three SBRs, the effluent from SBR fed with starch is more aromatic. Organic carbon with MW>1 kDa as well as the hydrophobic fraction in DOM gradually increased with the carbon sources changing from sodium acetate to glucose and starch. The DOC fractionation and the EEM all demonstrated that EfOM from the effluent of the SBR fed with starch contained more fulvic acid-like substances comparing with the SBR fed with sodium acetate and glucose.

关键词： effluent organic matter (EfOM)     extracellular polymeric substance (EPS)     molecular weight distribution (MWD)     hydrophobic     hydrophilic     excitation emission matrices (EEM)    

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Anaerobic digestion of sludge by different pretreatments: Changes of amino acids and microbial community

《环境科学与工程前沿（英文）》 2022年 第16卷 第2期 doi: 10.1007/s11783-021-1458-7

摘要：

•Tryptophan protein, and aromatic protein I/II were the key identified proteins.

关键词： Sludge pretreatments     Dissolved organic nitrogen     Proteins     Amino acids     Structural equation model     Metagenomic sequencing analysis.    

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Uncovering the effect of poly(ethylene-co-vinyl alcohol) molecular weight and vinyl alcohol content on

《化学科学与工程前沿（英文）》 2023年 第17卷 第10期   页码 1484-1502 doi: 10.1007/s11705-023-2331-y

摘要： Various hydrophilic poly(ethylene-co-vinyl alcohol) (EVOH) were used herein to precisely control the structure and hydrodynamic properties of polysulfone (PSF) membranes. Particularly, to prepare pristine PSF and PSF/EVOH blends with increasing vinyl alcohol (VOH: 73%, 68%, 56%), the non-solvent-induced phase separation (NIPS) technique was used. Polyethylene glycol was used as a compatibilizer and as a porogen in N,N-dimethylacetamide. Rheological and ultrasonic separation kinetic measurements were also carried out to develop an ultrafiltration membrane mechanism. The extracted membrane properties and filtration capabilities were systematically compared to the proposed mechanism. Accordingly, the addition of EVOH led to an increase in the rheology of the dopes. The resulting membranes exhibited a microporous structure, while the finger-like structures became more evident with increasing VOH content. The PSF/EVOH behavior was changed from immediate to delayed segregation due to a change in the hydrodynamic kinetics. Interestingly, the PSF/EVOH32 membranes showed high hydrophilicity and achieved a pure water permeability of 264 L·m–2·h–1·bar–1, which was higher than that of pure PSF membranes (171 L·m–2·h–1·bar–1). In addition, PSF/EVOH32 rejected bovine serum albumin at a high rate (> 90%) and achieved a significant restoration of permeability. Finally, from the thermodynamic and hydrodynamic results, valuable insights into the selection of hydrophilic copolymers were provided to tailor the membrane structure while improving both the permeability and antifouling performance.

关键词： polysulfone     blend modification     ultrafiltration membrane     formation hydrodynamics     poly(ethylene-co-vinyl alcohol) copolymer    

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Validation of polarizable force field parameters for nucleic acids by inter-molecular interactions

Liaoran Cao,Hong Ren,Jing Miao,Wei Guo,Yan Li,Guohui Li

《化学科学与工程前沿（英文）》 2016年 第10卷 第2期   页码 203-212 doi: 10.1007/s11705-016-1572-4

摘要： Modeling structural and thermodynamic properties of nucleic acids has long been a challenge in the development of force fields. Polarizable force fields are a new generation of potential functions to take charge redistribution and induced dipole into account, and have been proved to be reliable to model small molecules, polypeptides and proteins, but their use on nucleic acids is still rather limited. In this article, the interactions between nucleic acids and a small molecule or ion were modeled by AMOEBAbio09, a modern polarizable force field, and conventional non-polarizable AMBER99sb and CHARMM36 force fields. The resulting intermolecular interaction energies were compared with those calculated by ab initio quantum mechanics methods. Although the test is not sufficient to prove the reliability of the polarizable force field, the results at least validate its capability in modeling energetics of static configurations, which is one basic component in force field parameterization.

关键词： nucleic acid     polarizable force field     AMOEBA    

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Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on PersistentOrganic Pollutants (2008–2019)

《环境科学与工程前沿（英文）》 2022年 第16卷 第10期 doi: 10.1007/s11783-022-1541-8

摘要：

● Perfluorooctanesulfonic acid and perfluorooctanoic acid highest in human milk.

关键词： Human biomonitoring     Human breast milk     LC-MS/MS analysis     Lifestyle parameters    

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Effects of molecular weight and concentration of carboxymethyl cellulose on morphology of hydroxyapatite

Ruiqiang LIU,Rattan LAL

《环境科学与工程前沿（英文）》 2015年 第9卷 第5期   页码 804-812 doi: 10.1007/s11783-015-0785-y

摘要： Nano-sized apatite particles (nAP) synthesized with carboxymethyl cellulose (CMC) have shown great application potentials in in situ heavy metal remediation. However, differences in CMC’s properties effects on the size of nAP produced are not well understood. In this paper, two types of CMC, with respective molecular weights (MW) of ~120000 and ~240000 Dalton or respective polymerization degrees of 500 (CMC-500) and 1050 (CMC-1050), were studied in a concentration range of 0.05%–0.5% (w/w) for nAP synthesis. Morphology of the particles was characterized with transmission electron microscopy (TEM). Results showed that 0.05% CMC-500 solution gave an average particle size of 148.7±134.9 nm, 0.25% CMC-500 solution produced particles of 21.8±20.4 nm, and, 0.5% CMC-500 solution contained particles of 15.8±7.7 nm. In comparison, 0.05% CMC-1050 solution produced nanoparticles of 6.8±3.2 nm, 0.25% CMC-1050 produced smaller nAP of 4.3±3.2 nm, and 0.5% CMC-1050 synthesized the smallest nanoparticles in this study, with an average diameter of 3.0±2.1 nm. Chemical composition of the products was identified with X-ray diffraction (XRD) as pure hydroxyapatite. Interactions between nAP and CMC were discussed with help of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic data. This study showed that CMC at higher concentration as well as higher MW facilitated to produce finer nanoparticles, showing that nAP size could be manipulated by selecting appropriate CMC MW and/or applying appropriate CMC concentration.

关键词： nanotechnology     nanoparticles     apatite     carboxymethyl cellulose (CMC)     particle size    

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Low-carbohydrate diets lead to greater weight loss and better glucose homeostasis than exercise: a randomized

《医学前沿（英文）》 2021年 第15卷 第3期   页码 460-471 doi: 10.1007/s11684-021-0861-6

摘要： Lifestyle interventions, including dietary adjustments and exercise, are important for obesity management. This study enrolled adults with overweight or obesity to explore whether either low-carbohydrate diet (LCD) or exercise is more effective in metabolism improvement. Forty-five eligible subjects were randomly divided into an LCD group (n=22) and an exercise group (EX, n=23). The subjects either adopted LCD (carbohydrate intake<50 g/day) or performed moderate-to-vigorous exercise (≥30 min/day) for 3 weeks. After the interventions, LCD led to a larger weight loss than EX (−3.56±0.37 kg vs. −1.24±0.39 kg, P<0.001), as well as a larger reduction in fat mass (−2.10±0.18 kg vs. −1.25±0.24 kg, P=0.007) and waist circumference (−5.25±0.52 cm vs. −3.45±0.38 cm, P=0.008). Both interventions reduced visceral and subcutaneous fat and improved liver steatosis and insulin resistance. Triglycerides decreased in both two groups, whereas low-density lipoprotein cholesterol increased in the LCD group but decreased in the EX group. Various glycemic parameters, including serum glycated albumin, mean sensor glucose, coefficient of variability (CV), and largest amplitude of glycemic excursions, substantially declined in the LCD group. Only CV slightly decreased after exercise. This pilot study suggested that the effects of LCD and exercise are similar in alleviating liver steatosis and insulin resistance. Compared with exercise, LCD might be more efficient for weight loss and glucose homeostasis in people with obesity.

关键词： low-carbohydrate diet     obesity     nonalcoholic fatty liver disease     continuous glucose monitoring     mean sensor glucose    

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Tetra-detector size exclusion chromatography characterization of molecular and solution properties of

Qingbin Guo,Sheng Chang

《环境科学与工程前沿（英文）》 2017年 第11卷 第2期 doi: 10.1007/s11783-017-0922-x

摘要： Tetra-detector HPSEC was evaluated for the SMP characterization Molecular weight and intrinsic viscosity of the SMP were characterized Specific viscosity and osmotic pressure of the SMP solution were studied Approach to analyze the concentration polarization of the SMP was discussed Characterization of the molecular properties of soluble microbial products (SMP) is critical for understanding the membrane filtration and fouling mechanisms in anaerobic and aerobic membrane bioreactors (AnMBR & MBR). In this study, the distributions of the absolute molecular weight and intrinsic viscosity of SMP polysaccharides from an AnMBR were effectively determined by a high performance size exclusion chromatography (HPSEC) that was coupled with the refractive index (RI), diode array UV (DAUV), right and low angle light scattering (LS), and viscometer (Vis) detectors. Based on the tetra-detector HPSEC determined absolute molecular weights and intrinsic viscosity, a universal calibration relationship for the SMP polysaccharides was developed and the molecular conformations, average molecular weights, and hydrodynamic sizes of the SMP polysaccharides were also explored. Two factors which can be derived from the tetra-detector HPSEC analysis were proposed for the characterization of the viscous and osmotic pressure properties of the SMP polysaccharides. In addition, it was also extrapolated how to analyze the resistance characteristics of the concentration polarization layers formed in membrane filtration based on the molecular properties determined by the tetra-detector HPSEC analysis.

关键词： Soluble microbial product     Extracellular polymeric substance     Membrane bioreactor     Membrane fouling     Molecular weight     Intrinsic viscosity    

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vanbaalenii PYR-1 endowed with an enhanced activity of dihydroxylation of high-molecular-weight

Yiquan Wu, Ying Xu, Ningyi Zhou

《环境科学与工程前沿（英文）》 2020年 第14卷 第1期 doi: 10.1007/s11783-019-1193-5

摘要： • Mycobacterium vanbaalenii PYR-1 utilizes PAHs at different rates. • Both NidA3B3 and FNidA3B3 catalyze high-molecular-weight PAHs dihydroxylation. • NidA3B3 shows an enhanced activity with an endogenous electron transport chain. • FNidA3B3 has an enhanced activity by 50% approximately compared with NidA3B3. • FNidA3B3/NidA3B3 and NidAB in the same strain come from different ancestors. NidA3B3 is a terminal dioxygenase whose favorable substrates are high-molecular-weight polyaromatic hydrocarbons (PAHs) from Mycobacterium vanbaalenii PYR-1, a powerful PAHs degradation strain. NidA3B3 was reported to incorporate a dioxygen into the benzene ring of PAHs when equipped with an exogenous electron transport chain components PhdCD from Nocardioides sp. strain KP7 by biotransformation, but this enzyme system was not particularly efficient. In this study, strain PYR-1 was confirmed to utilize four different PAHs at different growth rates. When PhtAcAd, an endogenous electron transport chain of a phthalate dioxygenase system, was substituted for PhdCD to couple with NidA3B3, the specific activity to convert phenanthrene by strain BL21(DE3) [pNidA3B3-PhAcAd] was 0.15±0.03 U/mg, but the specific activity of strain BL21(DE3) [pNidA3B3-PhdCD] was only 0.025±0.006 U/mg. In addition, FNidA3, encoded by a newly defined ORF, has a prolonged 19-amino acid sequence at the N-terminal compared with NidA3. FNidA3B3 increased the activity by 50% approximately than NidA3B3 when using PhtAcAd. Components of the electron transport chain PhtAc and PhtAd were purified and characterized. The Km, kcat, kcat/Km values of the PhtAd were 123±26.9 M, 503±49.9 min−1, 4.1 M−1·min−1, respectively. And the Km, kcat, kcat/Km values of the ferredoxin PhtAc were 52.5±9.7 M, 3.8±0.19 min−1 and 0.07 M−1·min−1, respectively. Basing on the phylogenetic analysis, NidA3/FNidA3 were far from its isoenzyme NidA from the same strain. Combining their primary differences of transcriptional pattern in vivo, it indicated that the functionally similar Rieske dioxygenases NidA3B3/FNidA3B3 and NidAB might originate from different ancestors.

关键词： Biodegradation     polyaromatic hydrocarbons     biotransformation     ring-hydroxylating dioxygenase system    

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oxidizing environment on digestate humification and identification of substances governing the dissolved organic

《环境科学与工程前沿（英文）》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1520-0

摘要：

• Liquid digestate humification was investigated under different oxidizing environment.

关键词： Liquid digestate     MnO2     Oxygen     DOM transformation     Humic-like acids    

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Bile acids and their effects on diabetes

Cynthia Rajani, Wei Jia

《医学前沿（英文）》 2018年 第12卷 第6期   页码 608-623 doi: 10.1007/s11684-018-0644-x

摘要： Diabetes is a widespread, rapidly increasing metabolic disease that is driven by hyperglycemia. Early glycemic control is of primary importance to avoid vascular complications including development of retinal disorders leading to blindness, end-stage renal disease, and accelerated atherosclerosis with a higher risk of myocardial infarction, stroke and limb amputations. Even after hyperglycemia has been brought under control, “metabolic memory,” a cluster of irreversible metabolic changes that allow diabetes to progress, may persist depending on the duration of hyperglycemia. Manipulation of bile acid (BA) receptors and the BA pool have been shown to be useful in establishing glycemic control in diabetes due to their ability to regulate energy metabolism by binding and activating nuclear transcription factors such as farnesoid X receptor (FXR) in liver and intestine as well as the G-protein coupled receptor, TGR5, in enteroendocrine cells and pancreatic -cells. The downstream targets of BA activated FXR, FGF15/21, are also important for glucose/insulin homeostasis. In this review we will discuss the effect of BAs on glucose and lipid metabolism and explore recent research on establishing glycemic control in diabetes through the manipulation of BAs and their receptors in the liver, intestine and pancreas, alteration of the enterohepatic circulation, bariatric surgery and alignment of circadian rhythms.

关键词： bile acids     metabolic memory     diabetes     circadian rhythm     bariatric surgery    

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标题 作者 时间 类型 操作

Characterization of soil low-molecular-weight organic acids in the Karst rocky desertification region

Xiaoliang LI, Xiaomin CHEN, Xia LIU, Lianchuan ZHOU, Xinqiang YANG

期刊论文

Effects of a dynamic membrane formed with polyethylene glycol on the ultrafiltration of natural organic

Boksoon KWON, Noeon PARK, Jaeweon CHO,

期刊论文

Effects of organic acids on Cd adsorption and desorption by two anthropic soils

Jingui WANG, Jialong LV, Yaolong FU

期刊论文

Factors controlling

Chengkun WANG, Xiaojian ZHANG, Chao CHEN, Jun WANG

期刊论文

Characterization of the dissolved organic matter in sewage effluent of sequence batch reactor: the impact

Jin GUO, Feng SHENG, Jianhua GUO, Xiong YANG, Mintao MA, Yongzhen PENG

期刊论文

Anaerobic digestion of sludge by different pretreatments: Changes of amino acids and microbial community

期刊论文

Uncovering the effect of poly(ethylene-co-vinyl alcohol) molecular weight and vinyl alcohol content on

期刊论文

Validation of polarizable force field parameters for nucleic acids by inter-molecular interactions

Liaoran Cao,Hong Ren,Jing Miao,Wei Guo,Yan Li,Guohui Li

期刊论文

Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on PersistentOrganic Pollutants (2008–2019)

期刊论文

Effects of molecular weight and concentration of carboxymethyl cellulose on morphology of hydroxyapatite

Ruiqiang LIU,Rattan LAL

期刊论文

Low-carbohydrate diets lead to greater weight loss and better glucose homeostasis than exercise: a randomized

期刊论文

Tetra-detector size exclusion chromatography characterization of molecular and solution properties of

Qingbin Guo,Sheng Chang

期刊论文

vanbaalenii PYR-1 endowed with an enhanced activity of dihydroxylation of high-molecular-weight

Yiquan Wu, Ying Xu, Ningyi Zhou

期刊论文

oxidizing environment on digestate humification and identification of substances governing the dissolved organic

期刊论文

Bile acids and their effects on diabetes

Cynthia Rajani, Wei Jia

期刊论文